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Laboratory Robotics

Automation of AMPLICOR PCR using the Tecan Miniprep 75

[The following is a presentation given by Dr Jim Guthrie at IUSTI-Europe in Vienna, September 2002. Many thanks to Dr Guthrie, Southern General Hospital, Glasgow, Scotland, for permission to reproduce it here. The problem was how to meet the screening strategy envisaged by the Scottish Intercollegiate Guidelines Network, 2000. The presentation illustrates how laboratory robotics can be used to minimise the processing associated with nucleic acid based amplification assays; in this case the the Roche AMPLICOR® PCR. This can be achieved either by using automated assay equipment specified by the test manufacturer or, with a bit of ingenuity as here, by the adaptation of existing programmable equipment. Of course the objective of automation is to achieve higher, consistent specimen throughput and as exemplified in Fig 22, to leave the laboratory worker  with time for the newspaper 5 - V.Good ].

Guthrie1.GIF (6186 bytes) Guthrie2.GIF (8709 bytes) Guthrie3.GIF (8212 bytes) Guthrie4.GIF (9480 bytes)
Fig 1. Title. These figures © Guthrie et al., 2002. Fig 2. Chlamydia services provided by Southern General Hospital, Glasgow.. Fig 3. Screening strategy. Fig 4. The Scottish intercollegiate guidelines network.
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Fig 5. Implementation of Chlamydia screening strategy. Fig 6. The COBAS AMPLICOR ® Fig 7. Disadvantages of manual sample processing. Fig 8. Time for manually processing cervical swabs.
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Fig 9. Time for manually processing urines. Fig 10. The Tecan Miniprep 75. Fig 11. Picture of the Tecan Miniprep 75. Fig 12. Processing primary tubes.
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Fig 13. Urine and swab processing in deep well microtitre plates. Fig 14. Automated dispensing into A rings for the COBAS AMPLICOR. Fig 15. Key features of the Tecan Miniprep. Fig 16. Advantages of the Tecan Miniprep.
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Fig 17. Sample traceability via Tecan software. Fig 18. Time savings. Fig 19. Operator advantages. Fig 20. Impact on specimen throughput.
Guthrie21.GIF (6379 bytes) Guthrie22.GIF (115704 bytes)
Fig 21. Conclusions. Fig 22. Now for that newspaper !

Reference

Scottish Intercollegiate Guidelines Network: Management of genital Chlamydia trachomatis infection. March 2000.  Quick guideline (206 kb) [Acrobat] Full guideline (696kb)  [MEW comment: SIGN server either very slow or having a seriously bad day when I visited].

[MEW] November 2002.

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